A Microarray Analysis of the Host Response to Infection with Francisella tularensis
نویسنده
چکیده
................................................................................................................... 5 PAPERS IN THESIS..................................................................................................... 6 SAMMANFATTNING................................................................................................... 7 ABBREVIATIONS ........................................................................................................ 8 INTRODUCTION.......................................................................................................... 9 Microarray.................................................................................................................. 9 About the technology.............................................................................................. 9 Data analysis ......................................................................................................... 10 Francisella................................................................................................................ 13 History and classification...................................................................................... 13 The live vaccine strain .......................................................................................... 15 Tularemia .............................................................................................................. 15 Virulence and intracellular life of Francisella tularensis..................................... 17 Host response ........................................................................................................... 19 Innate immune response and pathogen recognition.............................................. 19 The role of ROS and RNS .................................................................................... 21 Response to intracellular pathogens ..................................................................... 23 Host immune response during tularemia .............................................................. 24 AIMS OF THE THESIS.............................................................................................. 27 RESULTS AND DISCUSSION .................................................................................. 29 Performance of different methods for microarray data analysis (Paper I)........... 29 Evaluation measures ............................................................................................. 29 Combined image analysis ..................................................................................... 31 Scanning procedures ............................................................................................. 31 Filtration................................................................................................................ 32 Background adjustment ........................................................................................ 32 Censoring .............................................................................................................. 33 Bias ....................................................................................................................... 33
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Microarray Analysis of Human Monocytes Infected with Francisella tularensis Identifies New Targets of Host Response Subversion
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